The relationship of certain types of human papillomavirus(HPV) infections and cervical cancers has been strongly confined. HPV types such as 6b and 11 are associated with benign tumors such as condylomas, while HPV types 16, 18, and occasionally,
31,
33, 51 types are associated with cervical intraepithelial neoplasia(CIN) and carcinoma. Especially HPV 16 and 18 types are frequently found in malignan tleisons of cervical tissues. In this study, we examined and compared the prevalence of
several
types
of HPVs in cervical tissues from Korean women using in situ hybridzation (ISH) and DNA polymerase chain reaction (PCR). Fifty cases of paraffin-embedded in only one of 14 cases with low and high grade CINs, HPV 16/18 types in 3(21%) of 14 with
low
and
high grade CINs, 6(24%) of 25 with CIS, and none of 4 with invasive carcinoma, and HPV types 31/33/51 in 2(14%) of 14 with low and high grade CINa, 6(25%) of 25 with CIS, and none of 4 with invasive carcinoma by ISH. Fifty-six cases of
paraffin-embedded
tissues and another 10 more tissues stored in liquid nitrogen were analyzed by PCR. When the presence of HPV 16 genome was examined by the amplification of 16 type E6 oncogene, 37/55(67%) and 6(60%) cases were positive for paraffin-embedded
tissues
and
frozen tissues, respectively. When the positivity for the presence of HPV 16 E6 genome in thirty-six tissues processed both for ISH, 9/36 (25%) and PCR, 26/36 (72%) was methods was compared, the latter was extremely sensitive, When the presence
of
HPV
L1 gene coding for major capsid antigen was amplified by using L1 consensus amplimers for paraffin-embedded tissues, 28 samples of 45(62%) were found to be positive, 21 of them were HPV 16-positive, while for frozen tissues 6 of 10 were HPV
16-positive.
This implies that other types of HPVs are presentin 7 and 2 cases of them, respectively. The amplified DNAs by PCR were confirmed by Southern blot hybridization using either 33P-labelled of biotin-labelled probe.
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